ADDITIONAL POLYMORPHIC MICROSATELLITE LOCI FOR DETAILED POPULATION GENETIC STUDIES OF LETHRUS APTERUS (COLEOPTERA: GEOTRUPIDAE)

  • Rita Rácz MTA-DE ‘Lendület’ Behavioural Research Group, H-4032 Debrecen, Egyetem tér 1, Hungary
  • Judit Bereczki MTA-DE ‘Lendület’ Behavioural Research Group, H-4032 Debrecen, Egyetem tér 1, Hungary
  • András Kosztolányi Department of Ecology, University of Veterinary Science Budapest H-1077 Budapest, Rottenbiller utca 50
  • Attila Horváth Genomic Medicine and Bioinformatic Core Facility, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen H-4032 Debrecen, Egyetem tér 1
  • Szabolcs Sziráki University of Debrecen, Faculty of Informatics H-4028 Debrecen, Kassai út 26
  • Zoltán Barta Department of Evolutionary Zoology and Human Biology, University of Debrecen H-4032 Debrecen, Egyetem tér 1, Hungary
DOI: https://doi.org/10.17109/AZH.63.3.355.2017
Keywords: dinucleotide repeats, trinucleotide repeats, fragmentation, species conservation, parentage

Abstract

In this study we attempted to isolate 13 additional microsatellites from the beetle Lethrus apterus and then to use them to screen for polymorphisms in 35 specimens collected from the northern Hungarian mountains. The newly developed markers presented in this pa-per are polymorphic, including between two and seven detected alleles per locus. The observed and expected heterozygosity per locus ranged between 0.057 and 0.686 and be-tween 0.056 and 0.756, respectively, while two loci showed significant deviations from Hardy-Weinberg equilibrium. These deviations can be explained by the presence of null alleles. All loci cross-amplified in at least two out of four congeneric Lethrus species (L. bituberculatus, L. scoparius, L. strymonensis and L. perun). The polymorphic microsatellite markers we present for L. apterus will be valuable for future population genetic studies.

Published
2018-12-15
How to Cite
RáczR., BereczkiJ., KosztolányiA., HorváthA., SzirákiS., & BartaZ. (2018). ADDITIONAL POLYMORPHIC MICROSATELLITE LOCI FOR DETAILED POPULATION GENETIC STUDIES OF LETHRUS APTERUS (COLEOPTERA: GEOTRUPIDAE). Acta Zoologica Academiae Scientiarum Hungaricae, 63(3), 355–360. https://doi.org/10.17109/AZH.63.3.355.2017
Issue
Vol. 63 No. 3 (2017): Acta Zoologica Academiae Scientiarum Hungaricae
Section
Article

Copyright (c) 2017 Rita Rácz, Judit Bereczki, András Kosztolányi, Attila Horváth, Szabolcs Sziráki, Zoltán Barta

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